TitleProduct

Elisa HDV AG Test

  • Price:

    Negotiable

  • minimum:

  • Total supply:

  • Delivery term:

    The date of payment from buyers deliver within days

  • seat:

    Beijing

  • Validity to:

    Long-term effective

  • Last update:

    2017-07-06 00:43

  • Browse the number:

    72

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Company Profile

Kantech International (Jiangxi) Co. Ltd

By certification [File Integrity]

Contact: egens-bio(Mr.)  

Telephone:

Area: Beijing

Address: Beijing

Website: http://egens-bio.sec66.com/

Product details
Model Number: ELISA HDV Ag Key Specifications/Special Features: Instructions for useHDV Ag ELISAASSAY procedure : :1. Add samples and extraction buffer:Open the foil pouch and remove the microplate. Dispensing 50μl of samples to each well, 50μl of negative control or 50μl of positive control to the respectivewells. Then dispensing 25μl of extraction buffer to each well except the blank well. Gently vibratingthe microplate. Cover and incubate for 60 minutes at 37℃.2. Wash the plate:Dilute 1 volume of wash buffer concentrate with 19 volumes of distilled water, mixwell. Remove the solutions from all the wells. Fill the wells with the diluted wash solution ( at least20 seconds to soak) then remove the diluted wash buffer from the wells. Repeat the procedure for5 times. Make sure that the rest volume is minimal, by blotting dry by tapping plate onto absorbentpaper.3. Add conjugate: Add the 50μl of conjugate to each well (except the blank well). Cover and incubatefor 30 minutes at 37℃.4. Wash the plate:Repeat the wash procedure as in step 2.5. Add 50μl of Substrate solution A and 50μl of Substrate solution B to each well, mix well. Coverand incubate for 10 minutes at 37℃.
6. Stop reaction:Add 50μl stop solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference
wavelength should be selected from 620nm to 690nm.
Results : :
1. For the assay to be valid, the average OD value of negative controls must be less than or equal to
0.1 and the average OD value of positive controls must be greater than or equal to 0.8.
2. Cut off value = 0.15 + average OD value of negative control
3. OD value of the sample ≥ cut off value, it is positive for HDV Ag
OD value of the sample < cut off value, it is negative for HDV Ag
Precautions : :
1. Allow all kit components to reach room temperature before use.
2. Follow the direction insert to control the reaction temperature and time strictly.
3. Do not mix components of different lot numbers to use.
4. The kit should be store at 2-8℃.Do not use kit components beyond their expiration date.
Shipping Information:
  • FOB Port: Shanghai
  • Lead Time: 20 - 25 days
Main Export Markets:
  • Asia
  • Australasia
  • Central/South America
  • Eastern Europe
  • Mid East/Africa
  • North America
  • Western Europe